Year: 2018 | Month: June | Volume 8 | Issue 3
Functional Characterization of Corpus Luteum and its Association with Peripheral Progesterone Profile at Different Stages of Estrous Cycle in the Buffalo
Abstract:
Corpus luteum (CL) serves as marker to ascertain the stages of cyclicity as well as to diagnose acyclic condition in the farm animals. Accordingly, we studied the cellular components and functional aspects of CL in relation to cyclicity with the buffalo ovaries (n=32) collected from slaughter house. The paired ovary samples (n=8, per stage) were categorized as early luteal (EL, day 1-5), mid luteal (ML, day 6-14) and late luteal (LL, day 15-21) and acyclic stage based on the gross ovarian dating. Histological changes of cellular differentiation of the CL were assessed using H&E staining and progesterone concentration in the luteal extract and peripheral circulation was measured by RIA method. A significantly higher (4.22 fold) proportion of small luteal cells were observed at ML (376.58±11.65) as compared to EL (89.35±5.54) and decreased thereafter in LL (57.40±3.02) stage. However, maximum number of large luteal cells was also found in ML (74.38±2.02) in contrast to EL (15.11±1.86) and LL (22.17±1.68). Further, serum and luteal progesterone was found maximum at ML stage and there was a significant (r=0.998) positive correlation. This study indicates maximum functional activity of CL was at ML stage in the buffalo.
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